Additional reminder for the use of color fluorescent beads: it has been found that green fluorescent beads are more ideal for young animals than for old animals. In addition, the tissue autofluorescence (background) of young animals is also lower. Therefore, if you can, please use young animals when doing reverse labeling experiments with green fluorescent beads.

 

 

 

The tracer product is highly concentrated in vivo injection, not easy to spread, strong signal, non-toxic to living cells or living bodies, and the survival time is more than one year. It is compatible with most of the following tracer, in situ hybridization detection technology and immunohistochemistry technology.

 

 

 

Since the excitation light segment of green fluorescent beads is shorter than that of red fluorescent beads, tissue autofluorescence is a problem. Therefore, the steps of reducing spontaneous fluorescence should be adopted to obtain better experimental results. These methods include: (1) using thinner sections, such as 30 μ m vs. 40 μ m or 50 μ m; (2) using young animals; (3) observing and photographing immediately after sealing (over time) The background will increase as well.